Antibacterial activity of green synthesized selenium nanoparticles using <i>Vaccinium arctostaphylos</i> (L.) fruit extract

In recent years, there has been an increase in the acceptance of green synthesis as a viable method for producing nanoparticles active materials that can inhibit bacterial resistance to antibiotics. The current study aimed evaluate effect synthesized selenium (SeNPs) using Vaccinium arctostaphylos L. fruit extract on Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Corynebacterium diphtheriae (C. diphtheriae). SeNPs were characterized by Ultraviolet-visible (U-V), Fourier-transform infrared (FTIR), Field Emission Scanning Electron Microscopy (FESEM), Dynamic Light Scattering (DLS), Zeta Potential (ZP) measurements. Minimum Inhibitory Concentration (MIC) Bactericidal (MBC) tested bacteria determined resazurin test, values used basis achieve antibacterial activity test detection viability (live/dead cell percentage) flow cytometry. this study, FESEM assay further shows Se NPs are precisely spherical uniform size (50 ± 1.23 nm) distribution. effectiveness against S. aureus, E. coli, C. diphtheria ranged from zero when MIC value was large (0.5 mg/ml) significant low (0.015625 mg/ml). high percentage death achieved diphtheria, 44.6%, 29.49%, 24.4% respectively. conclusion, V. desirable, safe, environmentally friendly options could be efficient agent prevent infections.